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Cell Line and Sample Identification

STRs (short tandem repeats) are repetitive sequence elements 3–7 base pairs in length scattered throughout the human genome. By amplifying and analyzing these polymorphic loci, and then comparing the resulting STR profile to that of a reference sample, the origin of biological samples such as cells or tissues can be identified and verified. The more loci that are amplified, the higher the statistical power of discrimination.

The GenePrint® Systems provide highly informative STR analysis for cell and tissue identification, human identity testing and mixed sample detection.

An Introduction to Cell Line and Sample ID

Short tandem repeat (STR) analysis is an informative approach to genetic identification that is commonly associated with DNA testing in forensic laboratories, paternity and missing persons cases. STR analysis is also used to verify tissue sample origins, authenticate cell lines, and detect tissue or cell mixtures.

STR profiling is a standard procedure for cell line authentication (ANSI/ATCC ASN-002). Cell line authentication provides researchers with confidence that their cell lines are correctly identified, and not cross contaminated with other cells. Major repositories now authenticate cell line identity, and many publications also require authentication of cell lines prior to publication of papers.

STR analysis is also used to confirm tissue identification. Any uncertainty as to a tissue’s origin must be resolved before the sample can be used in an experiment. STR analysis is a fast and easy way to do this. Researchers can compare the STR profile of the tissue with that of a reference sample to determine identity.

Likewise, STR analysis can be used to detect sample contamination, which appears as a mixed STR profile. The sensitivity of STR analysis, which can create full profiles from less than 100pg DNA, allows detection of minute quantities of contaminating cells or tissue.

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