Shipping Delays

UPS and FedEx are experiencing significant weather delays across the U.S. To maintain the high performance and reliability our customers expect, many orders received on or after February 10th may have been delayed for shipping. As inclement weather subsides and we resume shipping, you could expect a 1-3 day delay in transit. We apologize for any inconvenience this may cause.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Cloning and DNA Markers

Tools that modify nucleic acids provide the foundation for many molecular biology techniques such as subcloning.  For conventional cloning of DNA fragments, we provide a variety of restriction enzymes and T4 DNA Ligase. For bacterial transformation we offer a choice of competent cells, including our unique Single Step KRX strain. 

For other cloning applications we offer T4 DNA kinase, calf alkaline phosphatase and other modifying enzymes. For RNA transcription, T7, SP6 and T3 RNA polymerases are available.

An assortment of DNA/RNA markers including bench top and step ladders enable confirmation of a successful experiment.

Need a product modified?

Contact us to discuss custom size, formulation, concentration, packaging and format options for Promega cloning and modifying enzymes.

Learn More
Promega Custom Manufacturing Banner

Introduction to?Cloning and DNA Markers

Subcloning is a basic procedure in molecular biology that is used to move inserts from one vector to another to gain desired functionality and to characterize a DNA sequence of interest. 

One method to accomplish the transfer of a DNA insert uses restriction enzymes to digest both the fragment and the target vector, which is typically a plasmid. Confirmation of successful digestion is accomplished by comparing the expected size of the digested samples with DNA marker fragments. This is done by running samples on agarose gels to separate DNA fragments by size.  T4 DNA Ligase is then used to “paste” the desired fragment into the digested plasmid.

The next step is transformation. Transformation of bacteria with plasmids is important because bacteria are used as the means for both storing and replicating plasmids. E. coli cells are more likely to incorporate foreign DNA if their cell walls are altered so that DNA can pass through more easily. Such cells are said to be competent. Selection for cells that have been transformed successfully is done by antibiotic selection for the plasmid of interest.

There are many options to confirm if the transfer was successful including PCR, digestion with restriction enzymes or sequencing. Once confirmed, the recombinant vector can be used for a variety of applications such as protein expression or RNA transcription.

国产欧美日韩亚洲第一页_欧美人与动性行为视频_日韩视频中文在线一区_奇米影视777四色米奇影院 <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <文本链> <文本链> <文本链> <文本链> <文本链> <文本链>