Shipping Delays

UPS and FedEx are experiencing significant weather delays across the U.S. To maintain the high performance and reliability our customers expect, many orders received on or after February 10th may have been delayed for shipping. As inclement weather subsides and we resume shipping, you could expect a 1-3 day delay in transit. We apologize for any inconvenience this may cause.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Protein Detection

We offer a broad range of tools for detecting and quantifying proteins, from traditional immunological detection reagents to novel methods that monitor protein abundance in live cells.

Protein labeling products include HaloTag® Interchangeable Labeling Technology—a versatile protein analysis tool used to image live or fixed mammalian cells, purify proteins, investigate protein interactions and more. The HiBiT protein tagging system provides a powerful method for monitoring and quantifying proteins at endogenous expression levels with a simple luminescent signal.

Need a product modified?

Contact us to discuss custom packaging, formulation and format options for protein analysis products.

Learn More
Promega Custom Manufacturing Banner

Protein Quantitation and Detection Basics

The detection and quantitation of individual proteins is one of the fundamental aspects of proteomics. Immunological-based methods such as quantitative enzyme-linked immunosorbent assays (ELISA), Western blotting and dot blotting are very common and sensitive assays for protein detection, and they use antibodies that react specifically with entire proteins or specific epitopes (e.g., fusion tags) after cell lysis. Detection techniques are typically based on chemiluminescence or fluorescence.

For live-cell detection of proteins, fluorescence imaging microscopy is now routinely used. The most common and conventional method is the use of intrinsically fluorescent proteins (FPs) related in structure or sequence to green fluorescent protein (GFP). Alternatively, a series of self-labeling enzymes have been developed that can covalently attach a fluorescent ligand to one of its own amino acid residues. These enzymes are similar in size to FPs. If these enzyme domains are fused in frame to a protein, the pair can be labeled by introducing a cell-permeable fluorescent ligand, which covalently reacts with the fusion tag.

国产欧美日韩亚洲第一页_欧美人与动性行为视频_日韩视频中文在线一区_奇米影视777四色米奇影院 <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <文本链> <文本链> <文本链> <文本链> <文本链> <文本链>